TechNotes No. 7-2000
Binding of a plant lectin — horseradish peroxidase conjugate
to 2–acetamido–2–deoxy–a–D–galactopyranoside
GlycoWell™ plates.
Introduction
Binding of lectins to immobilized saccharides and subsequent
inhibition of such a binding event is often used for evaluating lectin inhibitors.
The use of GlycoWell™ plates facilitates an ELISA assay involving the binding
of lectins to the wells. This TechNote describes the binding of a 2–acetamido–2–deoxy–a–D–galactopyranoside
recognizing lectin, Maclura pomifera1, to 2–acetamido–2–deoxy–a–D–galactopyranoside
Glyco-Well™ plates.
Experimental
Discussion
The results demonstrate that a 2–acetamido–2–deoxy–a–D–galacto-pyranoside
GlycoWell™ plate efficiently binds a 2–acetamido–2–deoxy–a–D–galactopyranoside-recognizing
plant lectin, Maclura pomifera (Figure 1). Thus, GlycoWell plates are suitable
for detecting carbohydrate binding properties of proteins.
Materials
Figure 1. A Maclura pomifera-horseradish peroxidase conjugate binds to 2–acetamido–2–deoxy–a–D–galacto-pyranoside GlycoWell™ plates (SW-01-001), but not to a GlycoWell™ plate devoid of saccharide (SW-00-001). |